Use this url to cite publication: https://hdl.handle.net/20.500.12512/15542
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Experimental study of evaluation of articular cartilage chondrocyte viability in vitro / Tomas Mickevicius, Arvydas Usas, Alius Pockevicius, Sergej Sosunkevic, Rimtautas Gudas
Type of publication
Tezės kitame recenzuojamame leidinyje / Theses in other peer-reviewed publication (T1e)
Author(s)
Title
Experimental study of evaluation of articular cartilage chondrocyte viability in vitro / Tomas Mickevicius, Arvydas Usas, Alius Pockevicius, Sergej Sosunkevic, Rimtautas Gudas
Publisher (trusted)
Technologija |
Date Issued
Date Issued |
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2014-12-04 |
Extent
p. 79-81.
Is part of
International conference „Move for health 2014“ : Book of abstracts : [4 December 2014, Kaunas, Lithuania] / Lithuanian University of Health Sciences. Institute of Sports. Kaunas : Technologija, 2014. ISBN 9789955153733.
Version
Originalus / Original
Series/Report no.
Scientific session abstracts.
Field of Science
Abstract
Research background. Bone and cartilage complex (BCC) transplantation is faster and less expensive method of treatment of chondral lesions than chondrocyte transplantation. According to research studies athletes tend to recover faster after OATS surgery than after chondrocyte transplantation. Sometimes there are no possibilities to perform autologous BCC transplantation. The literature also scarce on data on chondrocyte viability after prolonged fraft storage under different temperature and culture media conditions. Research aim. Our hypothesis, chondrocyte viability in BCC directly affects integration of the transplanted BCC in allgraft recipients. In this study we seek to determine the optimal BCC storage conditions, which enable preservation of the most viable allograft. Research methods. 5 mm in diameter goat knee articular cartilage samples were kept under different storage conditions (n=8/per group): in the freezer at -70° C, in low glucose Dulbecco's Modified eAGLE'S mEDIUM (dmem) medium at +4° С or +37° C temperature. Samples were incubated for 14 and 28 days. The control group consisted of 8 samples harvested during surgery and stained on the same day. [...].
Type of document
type::text::conference output::conference proceedings::conference paper
ISBN (of the container)
9789955153733
Other Identifier(s)
(LSMU ALMA)990000856420107106
Coverage Spatial
Lietuva / Lithuania (LT)
Language
Anglų / English (en)
Affiliation(s)