Determination of terpenoids in Cannabis Sativa L. from North of Lithuania by Gas Chromatography Mass Spectrometry (GC-MS) after hydrodistillation
| Author(s) | |
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Bezruk, Ivan | National University of Pharmacy, Kharkiv, Ukraine |
| Date Issued |
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2019-04-10 |
Introduction. The primary research focus of Cannabis sativa L. are cannabinoids which present abundance indications for medical treatment, however. According to the literature sources, not only they responsible components for the activity of cannabis extracts. Terpenes play a significant role influencing some indications or inducing synergistic effects with cannabinoids. Terpenes shown many therapeutic effects like anti-inflammatory, anticarcinogen, sedative and plenty others. Terpenes and chemical compositions of Cannabis sativa L. crucially depends on its agricultural environment and breed. Since there are many different breeds, it is important to find out dominating terpenes in each breed. We analyzed two different breeds of Cannabis sativa L. obtained from Lithuanian north region. The aim of this research was to compare different breeds of Cannabis from Lithuania and determinate their terpenes composition. Materials and methods. Cannabis sativa L. breeds Santhica and Fedora were collected from Joniskis, Lithuania. Both materials were air–dried and grinded separately. For oils extraction from raw materials hydrodistillation was used. 15,0 g of material was mixed with 500,0 ml distilled water and boiled on the oil bath at 120˚ C for 3 h. Essential oils were diluted with 1,0 ml cyclohexan and then this solution was used for further GC-MS analysis. Terpene analysis were conducted using a Shimadzu GC-MS – QP2010 Ultra with AOC – 5000 Plus autosampler, Restek Rxi – 5ms (Restek Corporation) capillary column (30 m long with 0,25 mm outer diameter and 0,25 μm liquid-stationary phase thickness) with a liquid stationary phase (5% diphenyl and 95% polysiloxane). The instrument and operating conditions were: split injection mode with 60,0 split ratio, column oven temp 50,0˚ C, injection temp 260,00˚ C. The oven temperature was programmed from 50˚ C for 5 min, then 15,00 ˚C/min to 315,0˚ C and held constan[...].