Use this url to cite publication: https://hdl.handle.net/20.500.12512/36089
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Effects of Ginkgo biloba extract on heart and liver mitochondrial functions: mechanism(s) of action / Baliutyte G., Baniene R., Trumbeckaite S., Toleikis A
Type of publication
Konferencijų tezės nerecenzuojamame leidinyje / Conference theses in non-peer-reviewed publication (T2)
Title
Effects of Ginkgo biloba extract on heart and liver mitochondrial functions: mechanism(s) of action / Baliutyte G., Baniene R., Trumbeckaite S., Toleikis A
Date Issued
2010-04-22
Extent
p. 119.
Is part of
18th International Student Scientific Conference for Students and Young Doctors : Abstract book : Gdansk, Poland, 22-24 April 2010. .
Version
Originalus / Original
Field of Science
Abstract
Background: One of the possible applications of liposomes in medicine is an enhancement of reverse cholesterol transport mediated by high density lipoproteins (HDL). Interaction between HDL and liposomes generates new lipoprotein fraction migrating with pre-beta mobility in agarose gel. Aims: Here, we investigated the distribution of apo A-I and apo A-II in the pre-beta mobility fractions generated at liposomal-phosholipids/HDL-phospholipids (L-PL/HDL-PL) ratios 1:1, 3:1 and 5:1. Methods: Human serum containing HDL as a sole lipoprotein fraction and lecithin liposomes were incubated at 37C for 1 hour. Aliquots of incubation mixtures were analyzed by two-dimensional non-denaturing 2-27% polyacrylamide gradient gel electrophoresis (2D PAGGE). Pre-beta mobility fraction and intact liposomes were precipitated from incubation mixture with heparin and CaCl2 and then examined using non-denaturing 2-27% polyacrylamide gradient gel electrophoresis (PAGGE). Results: Immunoblots of 2D PAGGE of incubation mixtures revealed the presence of apo A-I and/or apo A-II containing lipoproteins migrating with pre-beta mobility. The range of Stokes diameters of apo A-I containing lipoproteins varied from 7.4 to 11.3 nm, with dominating fraction of diameters 8.3–11.3 nm. The diameters of apo A-II containing particles were mildly bigger and ranged from 8.7 to 13.4 nm. However, one could distinguish two main classes of particles with diameters 9.7–10.5 nm and 8.7–9.1 nm. Immunoblots of PAGE demonstrated that irrespectively of L-PL/HDL-PL ratio in incubation mixture, pre-beta mobility fractions were composed of similar classes of particles. Apo A-I containing population formed three species of particles with diameters from 12.4-10.0, 8.9 and 7.7 nm. On the other hand, apo A-II containing population included two bands of particles with diameters of 9.1 and 7.5 nm. Conclusions: Our results indicate, [...].
Type of document
type::text::conference output::conference proceedings::conference paper
Other Identifier(s)
(LSMU ALMA)990000137610107106
Coverage Spatial
Lenkija / Poland (PL)
Language
Anglų / English (en)