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The Interaction of hydrophobic bile acids with the alpha 1-proteinase inhibitor / S. Janciauskiene, S. Eriksson
Type of publication
Straipsnis Web of Science duomenų bazėje / Article in Web of Science database (S1a)
Author(s)
University Hospital Malmö, Malmö, Sweden | |
Eriksson, Sten | Lund University, Department of Medicine, Malmo General Hospital, Sweden |
Title
The Interaction of hydrophobic bile acids with the alpha 1-proteinase inhibitor / S. Janciauskiene, S. Eriksson
Publisher (trusted)
Is Referenced by
Date Issued
Date Issued |
---|
1994-04-25 |
Extent
p. 141-145.
Is part of
FEBS letters. Amsterdam : Elsevier Science, 1994, vol. 343, no. 2.
Version
Originalus / Original
Field of Science
Abstract
An in vitro complex formation between cholesterol and human alpha 1-proteinase inhibitor (alpha 1-antitrypsin, alpha 1-Pi) has been described. Hydrophobic bile acids were studied for a similar interaction using lithocholic acid (LC) as a prototype of a hydrophobic acid. At a molar ratio of 5:1, LC induced conformational changes of alpha 1-Pi reflected in an abnormal gel-electrophoretic appearance, loss of anodal immunoreactivity on crossed immunoelectrophoresis, exposition of new antigenic determinant(s) on immunodiffusion, and loss of antiproteinase activity. After 6 h incubation, LC and alpha 1-Pi form a complex of approximately 200 kDa molecular mass seen following gel-filtration. After prolonged (24 h) interaction a series of large alpha 1-Pi polymers were seen on SDS-PAGE under reducing conditions followed by Western blotting. Glycolitho-, sulfolitho-, deoxycholic and 3-beta-hydroxy-5-cholenoic acids induced similar but less pronounced changes of alpha 1-Pi, whereas transferrin remained unaffected. Hydrophilic acids lacked effect on alpha 1-Pi. The results are compatible with a specific, irreversible interaction of alpha 1-Pi with hydrophobic bile acids affecting its physical and proteinase inhibitory properties. The cholestatic potency of the hydrophobic acids studied and their ability to induce alpha 1-Pi polymerization may be important in cholestatic conditions.
Type of document
type::text::journal::journal article
ISSN (of the container)
0014-5793
WOS
A1994NJ16300009
Other Identifier(s)
(LSMU ALMA)990000380900107106
Coverage Spatial
Nyderlandai / Netherlands (NL)
Language
Anglų / English (en)