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The Effect of collagenase and temperature on mitochondrial respiratory parameters in saponin-skinned cardiac fibers / A. Toleikis, D. Majiene, S. Trumbeckaite, A. Dagys, A. Jasaitis
Type of publication
Straipsnis Web of Science duomenų bazėje / Article in Web of Science database (S1a)
Title
The Effect of collagenase and temperature on mitochondrial respiratory parameters in saponin-skinned cardiac fibers / A. Toleikis, D. Majiene, S. Trumbeckaite, A. Dagys, A. Jasaitis
Publisher (trusted)
Kluwer Academic/Plenum Publishing |
Is Referenced by
Date Issued
Date Issued |
---|
1996-06-01 |
Extent
p. 513-519.
Is part of
Bioscience reports. , 1996, vol. 16, no. 6.
Version
Originalus / Original
Description
pISSN 0144-8463,
Field of Science
Abstract
The results of a comparative study of the respiration rates of mitochondria in saponin-skinned rat cardiac fibers (SF) and in fibers treated with saponin and collagenase (SCF) suggest that only about half of the whole population of mitochondria manifest their activity in SF, in contrast to SCF, in response to extracellular substrates of oxidative phosphorylation. The apparent K-m value for ADP with succinate as substrate, which was as high as 330 +/- 32 mu M in SF in SF at 20 degrees C, decreased about 2-fold in SCF at the same temperature and in SF at 37 degrees C, and decreased further to 67 +/- 8 mu M in SCF at 37 degrees C. Thus, weakening or breaking of cellular contacts by collagenase and the temperature-dependence of diffusion of substrates such as ADP, seem to be important factors that determine the respiratory activity and regulatory parameters of mitochondria in saponin-permeabilized cardiomyocytes.
Type of document
type::text::journal::journal article
ISSN (of the container)
0144-8463
1573-4935
WOS
A1996WL42300007
Other Identifier(s)
(LSMU ALMA)990000419080107106
Coverage Spatial
Jungtinės Amerikos Valstijos / United States of America (US)
Language
Anglų / English (en)
Bibliographic Details
14