Interleukin-1B and IL1 receptor antagonist gene polymorphisms are not associated with premalignant gastric conditions: a combined haplotype analysis

Abstract

Objective: Contradictory results have been reported about the role of interleukin-1B (IL1B) and IL1 receptor antagonist (IL1RN) alleles in gastric carcinogenesis. Here, IL1B and IL1RN polymorphisms were analyzed as geno- and haplotypes in relation to the presence of atrophic gastritis (AG) and intestinal metaplasia (IM) in the stomach. Methods: Two-hundred-seventy-eight patients (212 Caucasians and 66 Asians) aged 50 years and older, referred for upper endoscopy due to dyspeptic symptoms, were included. Gastric biopsies were histologically assessed according to the updated Sydney classification. Genomic DNA was typed for polymorphisms at position -3737, -1464, -511, -31 for the IL1B gene and the allele 2 of IL1RN using restriction fragment length polymorphism of amplified PCR fragments and intron-spanning PCR analysis, respectively. Results: IL1B-1464-C/C genotype was associated with higher presence of AG in antrum of the stomach in Caucasians [OR: 4. 8 (95% CI = 1.7–14.3); p = 0.028]. IL1B-1464-G/C genotype was associated with lower incidence of AG in corpus of the stomach in Asians [OR: 0.7 (95% CI = 0.5–0.8); p = 0.02]. IL1RN*2 allele was not linked with AG or IM [...].