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Influence of AECOPD to ROS production in neutrophils / Mindaugas Vaitkus, Simona Lavinskiene, Kristina Bieksiene, Jolanta Jeroch, Raimundas Sakalauskas
Type of publication
Tezės kitoje duomenų bazėje / Theses in other database (T1c)
Title
Influence of AECOPD to ROS production in neutrophils / Mindaugas Vaitkus, Simona Lavinskiene, Kristina Bieksiene, Jolanta Jeroch, Raimundas Sakalauskas
Publisher (trusted)
Published for the European Respiratory Society by Maney Publishing
Date Issued
2011-09-24
Extent
p. 120s, no. P736.
Is part of
European Respiratory Journal : 21st Annual Congress Amsterdam, The Netherlands 24–28 September 2011: Abstracts / European Respiratory Society ; Editors: N. Ambrosino, I. Annesi-Maesano et al. London ; Cambridge, MA : Published for the European Respiratory Society by Maney Publishing, 2011, vol. 38, suppl. 55.
Version
Originalus / Original
Series/Report no.
89. COPD: human studies.
Field of Science
Abstract
Introduction and background: Chronic obstructive pulmonary disease (COPD) is characterized by specific pattern of inflammation involving polymorphonuclear neutrophils (PMN), macrophages and other cells. PMN are the major reactive oxygen species (ROS) producing cells. Our previous data showed that ROS production is decreased in stable COPD (SCOPD). It is still not known about ROS production in acute exacerbation of COPD (AECOPD). Aim: To investigate ROS production in PMN during AECOPD. Methods: 10 patients with SCOPD, 5 with AECOPD and 10 non-smoking healthy individuals (HI) were studied. PMN were isolated from peripheral blood using a high-density gradient. ROS production was induced using phorbol-myriste-acetate (PMA, 0.1-30 nM) and S. aureus bacteria (1-167 bacteria/neutrophil). ROS production was measured flow cytometrycally by mean of fluorescence intensity in PMN population. Results: The production of spontaneous ROS in PMN was 29.7±4.1% higher in AECOPD group than in SCOPD (p<0.05). PMA stimulated ROS production in all groups. The most significant increase of ROS production was observed between 0.3 and 1 nM PMA (AECOPD 172.5-fold, SCOPD 90.1-fold, HI 46.2-fold (p<0.05)). The higher ROS production in PMN after stimulation with different S. aureus concentration was found in AECOPD group compared with SCOPD and HI (p<0.05). Conclusion: Our data shows that ROS production is increased in AECOPD. Chemical (PMA) and biological (S. aureus bacteria) factors activates more intensive ROS generation.
Is Referenced by
Elsevier BIOBASE/Current Awareness in Biological Sciences
Biological Abstracts
BIOSIS Previews
Current Contents / Life Sciences
Current Contents / Clinical Medicine
Science Citation Index
SciSearch
Sociedad Iberoamericana de Informacion Cientifica (SIIC) Data Bases
CABI Global Health
Type of document
type::text::conference output::conference proceedings::conference paper
ISSN (of the container)
0904-1850
Other Identifier(s)
(LSMU ALMA)990000782190107106
Coverage Spatial
Nyderlandai / Netherlands (NL)
Language
Anglų / English (en)