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Oxidative stress related biomarkers in aged-related human studies on vitamin intervention and healthy aging cohorts
Jansen, Eugène H.J.M. | National Institute for Public Health and the Environment, Bilthoven, The Netherlands |
Beekhof, Piet K. | Laboratory for Health Protection research, National Institute for Public Health and the Environment, Bilthoven, The Netherlands |
Cremers, Johannes W.J.M. | Laboratory for Health Protection Research, National Institute for Public Health and the Environment, Bilthoven, The Netherlands |
| Date Issued |
|---|
2012-06-20 |
Oxidative stress and redox status have been related to many chronic diseases and ageing. To test whether biomarkers of oxidative stress really can describe the underlying pathological processes, large-scale screening is recommended in epidemiological cohorts. In addition many components of the oxidation and antioxidant system must be determined simultaneously to obtain an overall view of these processes. In this study we present an automated method for the determination of a large set of biomarkers of both oxidative processes and antioxidant status, which is suitable for large-scale screening. The following processes with representative biomarkers can be measured in large series: lipid peroxidation, protein oxidation, glycation, inflammation, tissue toxicity, iron status, total antioxidant status, anti oxidant enzymes and general serum antioxidants. The auto-analyser used for the measurements of oxidative stress and antioxidant biomarkers is the LX-20 Pro (Beckman-Coulter). The kits used on this auto-analyser are from various companies and have been adjusted to the format of analyzer. Applications will be shown of an age-related biomarker study of a strategic intervention study with multivitamin and mineral supplements (SOR/340006) and of a European cohort study on Health and Aging (CHANCES, EC-FP7).