Use this url to cite publication: https://hdl.handle.net/20.500.12512/89534
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Analysis of aloplastic cellulose-hydroxyapatite bone scaffold biological features / Julius Maminskas, Artūras Stumbras, Gintaras Juodžbalys, Jolanta Liesienė, Odeta Petrauskaitė, Pedro de Sousa Gomes, Maria Elisabete Jorge Vieira Costa
Type of publication
Konferencijų tezės nerecenzuojamame leidinyje / Conference theses in non-peer-reviewed publication (T2)
Author(s)
Liesienė, Jolanta | Kauno technologijos universitetas |
Petrauskaitė, Odeta | Kauno technologijos universitetas |
Gomes, Pedro de Sousa | Faculty of Dental Medicine, University of Porto, Porto, Portugal |
Costa, Maria Elisabete Jorge Vieira | Department of Materials and Ceramic Engineering, CICECO, University of Aveiro, Aveiro, Portugal |
Title
Analysis of aloplastic cellulose-hydroxyapatite bone scaffold biological features / Julius Maminskas, Artūras Stumbras, Gintaras Juodžbalys, Jolanta Liesienė, Odeta Petrauskaitė, Pedro de Sousa Gomes, Maria Elisabete Jorge Vieira Costa
Publisher (trusted)
Baltijos osteointegracijos akademija |
Date Issued
Date Issued |
---|
2012-09-07 |
Extent
p. 33, no. #31.
Is part of
Ketvirtasis tarptautinis BOA kongresas : programa ir pranešimų santraukos : 2012 m. rugsėjo 7-8 d., Kaunas, Lietuva = The Fourth International BOA Congress : Programme and Abstract Book : Kaunas, Lithuania 7-8 September, 2012 / Baltijos osteointegracijos akademija = Baltic Osseointegration Academy (BOA) ; BOA prezidentas/Prezident Gintaras Juodžbalys. Kaunas : BOA, 2012. ISBN 978-9955-905-11-0.
Version
Originalus / Original
Series/Report no.
Santraukos / Abstracts.
Santraukos / Abstracts.
Field of Science
Abstract
Objectives: Developing bone tissue engineering, a novel porous composite scaffold was made of manohydroxyapatite and carboxymethyl cellulose. The goal of this study is to evaluate biological properties of carboxyl- and non-carboxyl- nano-hydroxy-apatite/cellulose scaffolds in vitro and in vivo. Material and methods: Two groups of different scaffolds were used for in vitro and in vivo analysis. Both of them were cultivated 24 and 96 hours with rabbit mesenchymal cells in bovine serum medium containing antibiotics for evaluation in vitro. The same scaffolds were used in vivo and implanted subcutaneous for 10 mice (5 mice for each scaffold). After 14, 28, 56, 84 days of implantation, 2 mice were sacrificed in every period of time, and the scaffolds were harvested. The specimens were made and stained with H&E and Masson's thichrome and analyzed histologically. Results: Biocompatibility was confirmed with samples in vitro. The adhesion and proliferation of mesenchymal cells are observed microscopically in both scaffolds after 24 hours. After 96 hours, cells are proliferated only on surface of the non-carboxyl-nano-hydroxyapatite/cellulose scaffold. All mice stay alive after implantations of scaffolds. Light inflammatory response is observed in histological analysis after 14 days of implantation. Also, both of scaffolds are surrounded by fibrous and collagenous tissue with proliferation of connective tissue into the deeper structures of scaffolds. [...].
Type of document
type::text::conference output::conference proceedings::conference paper
ISBN (of the container)
978-9955-905-11-0
Other Identifier(s)
(LSMU ALMA)990000797630107106
Coverage Spatial
Lietuva / Lithuania (LT)
Language
Anglų / English (en)