Tissue engineering perspective in bone tissue regeneration: cellulose – hydroxyapatite composite scaffold
| Author | Affiliation |
|---|---|
Liesienė, Jolanta | Kauno technologijos universitetas |
Petrauskaitė, Odeta | Kauno technologijos universitetas |
Gomes, Pedro de Sousa | Department of Materials and Ceramic Engineering, CICECO, University of Aveiro, Aveiro, Portugal |
Costa, Maria Elisabete Jorge Vieira | Department of Materials and Ceramic Engineering, CICECO, University of Aveiro, Aveiro, Portugal |
| Date |
|---|
2013-09-13 |
Objectives: The goal of this study is to fabricate and investigate novel cellulose – hydroxyapatite composite scaffold for bone tissue engineering according to the bionic principle and to evaluate biological properties in vitro and in vivo. Material and methods: Cellulose gel was prepared by regeneration of cellulose from its acetylated derivates. porous cellulose matrix was created by freeze-drying method and covered with bioactive hydroxyapatite layer. Infrared spectroscopy, X-ray diffraction and scanning electron microscope were used to investigate ineractions between molecular components and scaffold surface morphology. Scaffolds were used for in vitro and in vivo analysis. Scaffolds were cultivated 24 and 96 hours with rabbit mesenchymal cells in bovine serum medium containing antibiotics for evaluation in vitro. [...].