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Type of publication: conference paper
Type of publication (PDB): Tezės Clarivate Analytics Web of Science ar/ir Scopus / Theses in Clarivate Analytics Web of Science and/or Scopus DB (T1a)
Field of Science: Medicina / Medicine (M001)
Author(s): Treinys, Rimantas;Mačianskienė, Regina;Almanaitytė, Mantė;Jurevičius, Jonas
Title: Alteration of action potential during early ischemia in the heart
Is part of: Biophysical journal : Biophysical society 61st Annual Meeting "Biophysics in the Big Easy" : February 11-15, 2017, New Orleans, Louisiana / Biophysical society. Cambridge, MA : Cell Press, 2017, vol. 112, iss. 3, suppl. 1
Extent: p. 401a-401a, no. 1981-Pos Board B301
Date: 2017
Series/Report no.: (Cardiac, smooth, and skeletal muscle electrophysiology II)
Note: This research is funded by a grant (No. MIP-58/2015) from the Research Council of Lithuania
Keywords: Myocardial ischemia;metabolism;Calcium channels, L-Type;metabolism;Calcium;metabolism;Oxidative phosphorylation;Action potentials;physiology;Rabbits
Abstract: Ischemic heart disease still is the most common cause of death in the world. Metabolic inhibition is typical for ischemic heart disease and heart failure and we investigated the effect of induced early ischemia and the changes of the action potential parameters in the isolated rabbit heart. In cardiac myocytes, L-type calcium current provides calcium for the activation of the contractile apparatus and is a crucial determinant of cardiac contractile activity. The suppression of L-type calcium current during ischemia is usually reported effect but our experiments revealed a dual respond of calcium current to the uncoupling of oxidative phosphorylation. When isoprenaline stimulated cardiac cells were exposed to FCCP, the uncoupler induced a biphasic effect on L-type calcium current. The exposure of the myocytes to FCCP evoked a rapid initial stimulation that was followed by a strong inhibition of L-type calcium current. We tested how this phenomenon is reflected in the action potential of isolated rabbit heart. Results showed that in the early phase of ischemia the action potential duration (APD) was briefly increased. At 20% repolarization APD increased up to 10% after 0.5 min of ischemia. This increase of APD was followed by a typical gradual decrease of APD when ischemia progresses. We attribute this dual change of APD to the biphasic changes of L-type calcium current and calcium transient increase in the early stage of ischemia in the heart
Affiliation(s): Lietuvos sveikatos mokslų universitetas
Membranų biofizikos laboratorija
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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