The Concentration of lactoferrin in cows milk in relation to health status of the udder
| Author(s) | ||
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Lietuvos sveikatos mokslų universitetas. Veterinarijos akademija. Virškinimo fiziologijos ir patologijos centras | ||
Lietuvos sveikatos mokslų universitetas. Veterinarijos akademija. Virškinimo fiziologijos ir patologijos centras | ||
Lietuvos sveikatos mokslų universitetas. Veterinarijos akademija. Virškinimo fiziologijos ir patologijos centras | ||
Lietuvos sveikatos mokslų universitetas. Veterinarijos akademija. Virškinimo fiziologijos ir patologijos centras | ||
Lietuvos sveikatos mokslų universitetas. Veterinarijos akademija. Virškinimo fiziologijos ir patologijos centras | ||
Lietuvos sveikatos mokslų universitetas. Veterinarijos akademija. Virškinimo fiziologijos ir patologijos centras | ||
Lietuvos sveikatos mokslų universitetas. Veterinarijos akademija. Virškinimo fiziologijos ir patologijos centras |
| Date Issued |
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2018-09-27 |
Konferencija skirta Lietuvos sveikatos mokslų universiteto Veterinarijos akademijos Gyvulininkystės technologijos fakulteto Gyvūnų auginimo technologijų instituto įkūrimo 80-čiui paminėti = The conference is devoted to the 80-th anniversary of Institute of Animal Rearing Technologies of Veterinary Academy of Lithuanian University of Health Sciences.
With the aim of facilitating the resistance of dairy cows against mastitis caused by pathogenic microflora, intensive investigations in immunology have been carried out almost for a century. Lactoferrin (LF) has been well recognized as multifunctional protein and one of the most important protective factors, which plays an important role in the prevention of mastitis infection at an early stage (Kawai et al., 2003; Wojdak-Maksymiec and Mikolajczyk, 2012). The aim of this study was to analyze an effect of udder‘s health status on concentration of LF in quarter milk samples (n=120) from crossbreed (Lithuanian Black-and-White) dairy cows. Milk samples from individual quarters (10 cows/40 quarter samples) were collected three times. The enzyme-linked immunosorbent assay (ELISA) used to determine LF in bovine biological fluids. The health status of the udder quarters was assessed according to microbiological analysis (MBA) and somatic cell count (SCC) in milk according Chaneton and others (2013). The quarters with the presence of mastitis caused by environmental microorganisms (EM), nonspecific mastitis (NM), or subclinical mastitis (SM) where classified as diseased quarters. The statistical analysis of the results were carried out using the SPSS version 15 (licence No. 9900457; SPSS Inc., Chicago, IL). A p≤0.05 was indicative of a statistically significant difference between groups. SCC and MBA results obtained in each group of quarters showed that LF concentration in the quarters with SM were on the average by 54% higher than in healthy quarters (0.10±0.01 mg/ml as against 0.03±0.01 mg/ml, p<0.001). It was detected higher LF concentration in quarters with EM (0.08±0.01 and 0.04±0.01 mg/ml, respectively) and quarters with NM (0.03±0.01 mg/ml, p<0.001) compared to healthy quarters.