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Type of publication: conference paper
Type of publication (PDB): Tezės kituose recenzuojamuose leidiniuose / Theses in other peer-reviewed publications (T1e)
Field of Science: Visuomenės sveikata / Public health (M004)
Author(s): Pudžiuvelytė, Lauryna;Jakštas, Valdas;Ivanauskas, Liudas;Marksa, Mindaugas;Bernatonienė, Jurga
Title: Apigenin extraction from fresh Elsholtzia ciliata herb
Is part of: Medicina : International Scientific Conference on Medicine, 77th International Scientific Conference of the University of Latvia : February 22, 2019, Riga, Latvia : abstracts / Editor-in-chief Edgaras Stankevičius. Kaunas ; Basel : LSMU ; MDPI, 2019, vol. 55, suppl. 1
Extent: p. 96-96 : lent
Date: 2019
Series/Report no.: (Poster presentations)
Keywords: Lamiaceae;chemistry;Plant extracts;analysis;Polyphenols;analysis;Apigenin
Abstract: Background. Polyphenols have gained a significant importance as bioactive compounds with substantial health benefits. The main properties of polyphenols are anti-diabetic, cardioprotective, anti-aging, neuroprotective, anti-inflammatory, antioxidative effects. The genus Elsholtzia from Lamiaceae family is rich in phenolic compounds. Elsholtzia ciliata is an annual aromatic plant used as a spice in cuisine and as a remedy in folk medicine. Aim. The aim of this study was to increase the extraction efficiency of apigenin from E. ciliata fresh herb. Methods. Extracts of fresh E. ciliata herb were produced using 96, 90 and 70% ethanol by ultrasound-assisted (UAE) (10 min, 25 °C), heat-reflux (HRE) (6 h, 95°C), continuous stirring (CSE) (24 h, 25 °C), percolation (PE) (48 h, 25 °C) and maceration (ME) (48 h, 25 °C) methods. HPLC analyses have been carried out using Waters 2695 chromatography system (Waters, Milford, CT), equipped with Waters 996 PDA detector. For analysis an ACE 5 C18 250 x 4.6 mm (Waters, Milford, USA) column was used. The mobile phase consisted of solvent A (phosphoric acid/acetonitrile/ water) (1:19:80 V/V/V) and solvent B (phosphoric acid/methanol/acetonitrile) (1:40:59 V/V/V). The linear gradient elution profile was as follows: 100 % A – at 0 min, 55 % A/45% B – at 20 min, 100 % B at 25 – to 26 min, 100 % A – at 30 – to 31 min. The flow rate was 1.2 mL/min and injection volume was 10 μL. Absorption was measured at 330 nm. Quantification of apigenin was performed using reference standard of apigenin. The linear calibration curve were constructed (R2 ==0.999979). Results. The lowest content of apigenin was obtained by ME and PE using 96% ethanol (141.06±1.7 and 161.51±1.43 μg/g, respectively). The highest content of apigenin (855.54±5.75 μg/g) was obtained by UAE, using 70% ethanol. During CSE, the content of apigenin depended on the solvent concentration used: 96 % ethanol w[...]
Affiliation(s): Analizinės ir toksikologinės chemijos katedra
Farmacijos mokslinė laboratorija
Farmakognozijos katedra
Lietuvos sveikatos mokslų universitetas
Vaistų technologijos ir socialinės farmacijos katedra
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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