Use this url to cite publication: https://hdl.handle.net/20.500.12512/99008
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Cytosolic fatty acid synthesis and mitochondrial fatty acid oxidation coexist simultaneously in breast cancer cells / Ieva Ceslevičienė, Valeryia Mikalayeva, Ieva Sarapinienė, Vytenis Arvydas Skeberdis, Vaidotas Žvikas, Sergio Bordel Velasco
Type of publication
Tezės kitame recenzuojamame leidinyje / Theses in other peer-reviewed publication (T1e)
Author(s)
Title
Cytosolic fatty acid synthesis and mitochondrial fatty acid oxidation coexist simultaneously in breast cancer cells / Ieva Ceslevičienė, Valeryia Mikalayeva, Ieva Sarapinienė, Vytenis Arvydas Skeberdis, Vaidotas Žvikas, Sergio Bordel Velasco
Publisher (trusted)
University of Latvia Press |
Date Issued
Date Issued |
---|
2019-06-17 |
Extent
p. 64-64, no. 8.
Is part of
FEBS3+ conference of Latvian, Lithuanian and Estonian biochemical societies : abstract book : riga, Latvia, 17-19 June 2019 / Organizers: Kaspars Tärs, Tiit Lukk, Daumantas Matulis, Zane Kalniņa, Nils Rostoks. Riga : University of Latvia Press, 2019. ISBN 9789934184505.
Version
Originalus / Original
Series/Report no.
Poster sesion (session VIII).
Description
ISBN 978-9934-18-450-5.
Field of Science
Abstract
The survival and proliferation of cancer cells depends on fatty acid synthesis and oxidation. The aim of this study was to evaluate existence of simultaneous cytosolic fatty acid synthesis (FAS) and mitochondrial fatty acid oxidation (FAO) In breast cancer cells. Metabolic flux analysis using 13Clabeled glutamine in combination with gene silencing using small interfering RNAS (siRNAs) against FASN, ECHS1 and ME genes. The simultaneous occurence of both processes implies that part of the acetyl-CoA feeding the TCA cycle is derived from citrate production in 5 labeled carbons (MS isotopomer). If these lipids are being simultaneously degraded, labeled acetyl-CoA will be entering the TCA cycle, resulting in the formation of fully labeled citric acid molecules (M6 isotopomer). The results showed that BCC (breast cancer cells) had the higher amount of fully labelled citrate (M6 isotopomer), than MCF7 (human breast adenocarcinoma cell line) and BT-474 (human breast ductal carcinoma cell line) cells. [...].
Type of document
type::text::conference output::conference proceedings::conference paper
ISBN (of the container)
9789934184505
Other Identifier(s)
(LSMU ALMA)990000988990107106
Coverage Spatial
Latvija / Latvia (LV)
Language
Anglų / English (en)
Affiliation(s)