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Quantitative composition of quercetin glycosides in e. ciliata ethanolic extracts obtained from different plant parts
| Date Issued |
|---|
2019-08-05 |
ISBN 978-9955-15-620-8.
Bibliogr.: p. 38
Introduction. Herbs have been traditionally used to treat or prevent various illnesses. Plants accumulate bioactive compounds such as phenolics, alkaloids, terpenoids, and sulfur-containing compounds (1,2). Various studies have concluded that the supposed beneficial effects of polyphenols are frequently related to their antioxidant activity that could have preventive or therapeutic effects for cardiovascular diseases, neurodegenerative disorders, cancer, and obesity (2-6). Elsholtzia ciliata – an annual plant belonging to Lamiaceae family (7). Phenols belong to the largest group of secondary metabolites in plants, foremost of the family Lamiaceae, and they exhibit multidirectional biological activity (8). Research aim. According to scientific literature, E. ciliata is a valuable bioactive source of natural antioxidants (9). The objective of this study was to determine the main phenolic compounds in the ethanolic extracts of E. ciliata leaves, stems, flowers, and whole herb. Research methods and organization. E. ciliata (Thunb.) Hyl. flowers, leaves, stems and whole plant were purchased from „Zolynu namai", Vilnius, Lithuania. All the plant parts were separated by hands and leave in a dry, dark place for 5 days to dry. Dried flowers, leaves, stems and whole herb separately were grounded in a laboratory mill. All the standards, reagents and solvents used through experiments were of analytical grade. Hyperoside, rutin, quercitrin, p-coumaric acid, caffeic acid, rosmarinic acid, and chlorogenic acid standards were purchased from Extrasynthese (Genay, France), avicularin from Chromadex (Santa Ana, USA). Acetic acid and acetonitrile and were obtained from Sigma-Aldrich GmbH (Buchs, Switzerland) and ethanol from Vilniaus degtine, AB (Vilnius, Lithuania). Powdered material of dried E. ciliata plant parts (each for 1 g) were extracted with 20 mL of 70% (v/v) (1:20) ethanol in a flask by ultrasound-assisted extraction per