Fabrication of scaffolds for stem cells via ultrafast laser micro machining of polymer films and electrospun webs

Abstract

Aim: Scaffolds, i.e. structures that allow controlling the behaviour of the cells, require advanced fabrication techniques that enable realization of complicated 3D micro structures. Ultrafast laser processing methods are widely applied, especially multi-photon polymerization techniques. The main drawback of the mentioned method is the limited range of materials and involved processes that are not always biocompatible with live organism. Methods: In the current work different approach was applied where top down lithography method - laser drilling of biocompatible material together with bottom-up method: electrospinning technique which allows obtaining a fibrous structure mimicking the extracellular matrix, was demonstrated. Results: Using ultrafast 1030 nm wavelength Yb:KGW femto-second laser and micro-fabrication workstation thin biocompatible polymer films were pre-structured with micrometer and tens or micrometer ranges membrane and grid structures. 3D scaffolds, i.e. layer of micro-/nano-fibers, were obtained employing electrospinning of different polymer composition solutions. Conclusions: Fabricated scaffolds provides suitable environment for the s