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Kliniškai sveikų kalių makšties citologiniai pokyčiai rujos metu
Baranauskaitė, Akvilė |
Žilaitis, Vytuolis |
Laurinavičiūtė, Vilija | Komisijos sekretorius / Committee Secretary |
Mockeliūnas, Raimundas | Komisijos pirmininkas / Committee Chairman |
Gintautas, Jonas | Komisijos narys / Committee Member |
Vaitkus, Valdas | Komisijos narys / Committee Member |
Ružauskas, Modestas | Komisijos narys / Committee Member |
Pockevičius, Alius | Komisijos narys / Committee Member |
Bakutis, Bronius | Komisijos narys / Committee Member |
Matusevičius, Algimantas Petras | Komisijos narys / Committee Member |
Darbo tikslas: Įvertinti kliniškai sveikų kalių makšties citologinius pokyčius prieš lytinę dominantę ir po jos. Darbo uždaviniai: 1. Apibendrinti literatūrinius duomenis apie kalių makšties citologinius pokyčius ir jų sąsają su lytinio ciklo stadija ir optimalaus kergimo laiku. 2. Įvertinti makšties citologinius pokyčius priklausomai nuo rujos stadijos. 3. Įvertinti makšties epitelio citologinių pokyčių priklausomybę nuo kalės dydžio. Išvados: 1. Priklausomai nuo rujos stadijos kinta makšties epitelio suragėjimo laipsnis. Priešrujo metu kyla suragėjimo laipsnis ir rujos pradžioje pasiekia 80 proc., o porujo pradžia nustatoma tuomet kai įvyksta staigus suragėjimo laipsnio kritimas. Kai suragėjimo laipsnis siekia 80 proc. ir daugiau, tai laikoma optimaliu kergimo laiku. 2. Ryškiausiai citologiniai pokyčiai vyksta priešrujo ir porujo metu, o rujos metu citologinis vaizdas išlieka pastovus. Lytinio ciklo laikas daugiausiai įtakos turi parabazinėms (26 proc.), paviršinėms epiletinėms ląstelėms (23 proc.) ir eritrocitams (26 proc.) (p<0,001). 3. Kalių dydis neturi įtakos makšties citologiniams pokyčiams, nes stebimi nedideli ląstelių kiekio skirtumai priklausomai nuo rujos ciklo laiko (p<0,05).
In particular periods of the year the bitches exhibit 1 – 2 estruses. This frequency depends on specific factors like breed, management conditions ant etc. The sexual cycle of bitches composed of proestrus, estrus, metestrus and anestrus stages. The increased concentration of estrogen during the proestrus period causes the vaginal epithelium proliferation and cornification. Vaginal cytology changes are very useful to determine canine estrus cycle stages and ovulation time. Vaginal cytology is a simple and inexpensive procedure that allows us to determine the stage of the reproductive cycle and ovulation time in bitches. Investigations were carried out 2011 – 2012 years. The aim of this study was to determine vaginal cytology changes during different stages of the sexual cycle of the clinical healthy bitches. A total of 15 (5 small breeds, 5 medium breeds and 5 large breeds) clinical healthy bitches, belonging to different breeders and owners, have been examined. Only sexual mature bitches were used in this study. The samples were begin collected at the time of appearance of vulvar bloody discharge (it was considered as the first day of proestrus) in series 25 days. The samples were collected using cotton swab technique. The cotton was moistened with 1 – 2 drops of the sterile NaCl saline (0.9%). The moistened swab was introduced at the most dorsal point of the vulvar cleft and angled upward at a 45–degree angle. The swab was rolled between fingers in the deepness of the vagina and pulled straight out. The swab was rolled several times over a clean glass slide. The prepared smears were air–dried and stained with May Grunwald – Giemsa stain. The vaginal smear samples were evaluated using light microscope. Neutrophils, erythrocytes and all of the vaginal epithelium cells (parabasal, intermediate and superficial) were counted. The statistical analysis of the data shows that breed of the bitches has not influence on vaginal cytology changes (p<0.05). The time of the reproductive cycle has the most influence on number of parabasal, superficial epithelium cells and erithrocytes (p<0.001). The most vaginal cytology changes were determined on proestrus and metestrus. Whan cornified cells index reaches more than 80 percents it is optimal time for breeding.