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Micro- and macroelements in seminal plasma influence dilutedboar semen quality for seven days
Bibliogr.: p. 72
Growing evidence shows that macro- and micro elements in the seminal plasma of domestic animals are of great importance due to their roles in sperm metabolism, function, survival and oxidative stress (1, 2). The current study was carried out to investigate the effect of micro- and macro elements in fresh boar seminal plasma and their association with sperm quality parameters after 7 days of liquid storage at 16°C. Semen samples were collected from 40 boars and diluted with a long-term extender at a final concentration of 35 × 106 sperm cells/mL. In the laboratory, the semen was assessed for sperm morphology, viability (eosin-nigrosin staining), pH,subjective and objective sperm motility by sperm class analyzer (SCA). Only those samples having more than 75% motility and more than 75% normal sperm were used for further experiments. Sperm viability, motility and pH were checked after 24, 48, 72 and 168 h of storage at 16°C. Seminal plasma was separated and the concentration of macro elements (Na, K, Ca, Mg, P) and micro elements (Cu, Zn, Fe) was determined. The longer storage time showed the lower sperm motility and viability results in all samples. Motility measured subjectively dropped by 21% (P < 0.01) and by 12.58% (P < 0.01) measured objectively. Viability decreased by 21.98% (P < 0.01) and pH value dropped by 0.21 (P < 0.01) after 7 days of incubation. Elements correlated with sperm quality parameters. Iron and copper negatively correlated with sperm tail abnormalities (P < 0.05)and showed a positive effect on sperm motility and viability results (P < 0.05) after 7 days. With the increase of potassium, sperm viability after 7 days declined (P < 0.01). After 7 days of storage, pH values strongly correlated significantly with sperm viability (P < 0.01). Our data showed that micro- and macro elements in seminal plasma have an effect on diluted boar semen quality parameters for 7 days of incubation and could be as a predictive value of boar semen fertility.