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Bradford method for microcystis biomass protein quantification
Type of publication
Recenzuojamos išplėstinės tezės / Peer-reviewed extended theses (T1d)
Author(s)
Karosienė, Jūratė | Gamtos tyrimų centras |
Koreivienė, Judita | Gamtos tyrimų centras |
Kasperovičienė, Jūratė | Gamtos tyrimų centras |
Title
Bradford method for microcystis biomass protein quantification
Neda Pašukonytė, Gabrielė Balčiūnaitė, Nijolė Savickienė, Jūratė Karosienė, Judita Koreivienė, Jūratė Kasperovičienė, Evelina Noreikaitė
Publisher (trusted)
Student’s Scientific Society of Lithuanian University of Health Sciences |
Date Issued
Date Issued |
---|
2020-11-19 |
Extent
p. 174-176.
Is part of
Health for All: 2020 - International Students’, Doctoral and Residents’ Conference: Science for Health : abstract book : [19-20 November, 2020, Kaunas, Lithuania] / [organised: Student’s Scientific Society of Lithuanian University of Health Sciences ; Edited by Monika Jasinskaitė, Karolina Slapšytė, Gvidas Mikalauskas]. Kaunas : Student’s Scientific Society of Lithuanian University of Health Sciences, 2020.
Version
Originalus / Original
Description
Posters
The Conference is organized by the Student’s Scientific Society of Lithuanian University of Health Sciences The content of the abstract book is redacted. The Student’s Scientific Society of Lithuanian University of Health Sciences did not have control over the abstracts. The authors remain responsible for the content of their respective abstracts. All abstracts presented in the conference were reviewed.
Bibliogr.: p. 176
Field of Science
Abstract
Introduction It is known, that microalgae are considered a viable source of protein and some of the species of microalgae contain similar protein levels as meat, eggs, soybeans and milk [1]. Aim The aim of the study – to determine the quantity of protein extracted from cyanobacteria biomass collected in Lake Simnas using Bradford test. Methods Cyanobacteria scum biomass from Lake Simnas (Alytus district) containing 84% of Microcystis and 12% of Dolichospermum species was received from the Nature Research Centre, Laboratory of Algology and Microbial Ecology. Wet weight of cyanobacteria biomass was poured into 15 mL test-tubes. Extraction was carried out by ultrasound sonication (3 times × 1min). Biomass homogenisation followed by pH adjustment to 13 with 1.0 M NaOH solution. The sample was cooled and centrifuged. Precipitate was discharged and supernatant was used in further experiments. Proteins were precipitated by TCA/Acetone, ice cold (-20 ºC) 13.3 % TCA solution in acetone, with 0.2 % of β mercaptoethanol (β-ME). Sample was incubated in -20 °C for 18 h. After incubation, sample was centrifuged and supernatant poured out. Precipitated proteins were washed with acetone and 0.2% β-ME. Washing was repeated 2 times. Washed protein pellets were resuspended in PBS buffer, pH 7.4. Bradford assay was used for protein quantification [2]. 595 nm wavelength absorption was measured with spectrophotometer [3]. Research results were statistically processed by using MS Excel software....[...].
Type of document
type::text::conference output::conference proceedings::conference paper
Other Identifier(s)
(LSMU ALMA)990001024750107106
Coverage Spatial
Lietuva / Lithuania (LT)
Language
Anglų / English (en)
Bibliographic Details
3