Use this url to cite publication: https://hdl.handle.net/20.500.12512/109907
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Determination of phenolic profile in Hippophae rhamnoides L. leaves extracts / Niekytė A., Raudonė L
Type of publication
Tezės kitame recenzuojamame leidinyje / Theses in other peer-reviewed publication (T1e)
Title
Determination of phenolic profile in Hippophae rhamnoides L. leaves extracts / Niekytė A., Raudonė L
Publisher (trusted)
Національний фармацевтичний університет |
Date Issued
Date Issued |
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2021-04-02 |
Extent
p. 31-32.
Is part of
Сучасні досягнення фармацевтичної науки в створенні та стандартизації лікарських засобів і дієтичних добавок, що містять компоненти природного походження : Матеріали ІІІ Міжнародної науково-практичної інтернет-конференції : 2 квітня 2021 року м. Харків = Современные достижения фармацевтической науки в создании и стандартизации лекарственных средств и диетических добавок, которые содержат компоненты природного происхождения : Материалы ІІІ Международной научно-практической интернет-конференции = Current approaches of pharmaceutical science in development and standardization of medicines and dietary supplements that contain components of natural origin : The Proceedings of the ІІI International Scientific and Practical Internet-Conference. Харків / Харьков / Kharkiv : НФаУ, 2021.
Version
Originalus / Original
Description
Bibliogr.: p. 31-32
Field of Science
Abstract
Introduction. Sea buckthorn (Hippophae rhamnoides L. family of Elaeagnaceae) leaves are an excellent source of bioactive compounds, such phenolic acids, tannins and flavonoids, which have various beneficial effects on health, such as strong antioxidant, anticancer, anti-inflammatory and antibacterial effects [1,2]. Studies showed that different developmental stages during the plant life cycle affects the content of secondary plant metabolites and their distribution between different morphological parts of the plant [3]. The aim of this study is to determine qualitative and quantitative composition variability of phenolic compounds in sea buckthorn leaf extracts during vegetation. Materials and Methods. Hippophae rhamnoides L. leaf samples were collected in their natural habitat in the fields near the town of Žeimelis (coordinates: 56°15'48.3"N 23°59'26.3"E). They were collected in different vegetation periods from May to the end of September during the year 2019. The extracts were prepared using 0,2g of dry matter and 20 ml of 70% ethanol, and the extraction was performed in an ultrasonic bath for 15 minutes. The phenolic composition of seabuckthorn leaf extract was determined by HPLC-PDA method, using the ACE C18 reversed-phase column in the solvent system with 0.1% trifluoroacetic acid and acetonitrile under gradient elution. The analyte and reference compound retention time and UV absorption spectra were used for peak identification. Results and their discussion. Were identified and quantified 13 phenolic compounds, of which 4 phenolic acids (gallic, protocatechuic, caftaric, ellagic), 3 flav-3-ols (catechin, epigallocatechin and epicatechin gallate), 4 flavonoids (rutin, isoramnetin-3-rutinoside), isoramnetin-3-glucoside, quercetin-3-O-(6''-acetyl-glucoside)), 1 glycosylated flavonoid - tiliroside and a proanthocyanidin group representative - procyanidin B3. Epigallocatechin,[...].
Type of document
type::text::conference output::conference proceedings::conference paper
ISSN (of the container)
2519-2655
Other Identifier(s)
(LSMU ALMA)990001032680107106
Coverage Spatial
Ukraina / Ukraine (UA)
Language
Anglų / English (en)
Bibliographic Details
3