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Distribution of cholinergic and adrenergic neurons in the heart of a frog / Greta Graužinytė, Agnė Andriuškevičiūtė; Supervisor of the abstract: Gertrūda Skripkienė
Type of publication
Recenzuojamos išplėstinės tezės / Peer-reviewed extended theses (T1d)
Title
Distribution of cholinergic and adrenergic neurons in the heart of a frog / Greta Graužinytė, Agnė Andriuškevičiūtė; Supervisor of the abstract: Gertrūda Skripkienė |
Publisher (trusted)
Lietuvos sveikatos mokslų universiteto studentų mokslinė draugija |
Date Issued
Date Issued |
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2015-05-06 |
Extent
p. 277-279.
Is part of
International Health Sciences Conference 2015, [3rd : 6th to 8th of May, 2015 in Kaunas] : Abstract book / Students‘ Scientific Society of Lithuanian University of Health Sciences ; [Edited by: Artūras Jacinavičius, Edvardas Naujalis, Mindaugas Stankevičius, Karolis Varkalys]. Kaunas : Lietuvos sveikatos mokslų universiteto studentų mokslinė draugija, 2015.
Version
Originalus / Original
Series/Report no.
Posters session.
Description
Bibliogr.: p. 279
Field of Science
Abstract
Introduction The intrinsic cardiac nervous system plays an important role in the regulation of heart activity (2). A frog is a convenient animal model for anatomical and physiological studies of the intracardiac neurons (1, 4). Compared to a mammalian heart the intracardiac neurons of the frog is more accessible for researchers (1). Although the architecture of the intracardiac nerve plexus and the morphological characteristics of the cardiac ganglion nerve cells of the frog have been studied (1), little is known about the immunohistochemistry of cardiac neurons. Up to date, the distribution of the cholinergic and adrenergic neurons in the frog intracardiac nerve plexus has not been evaluated. This work was performed in order to assess the distributive features of neurons in the heart of a frog. Aim The purpose of this study is to determine the distribution of cholinergic and adrenergic neurons in intact intracardiac nerve plexus of the frog using double immunohistochemical labeling. Objectives 1. To visualize cholinergic and adrenergic neurons in flattened intact preparation of the heart of the frog using double immunohistochemical labeling. 2. To determine the distribution of cholinergic and adrenergic neurons in the intracardiac nerve plexus of the heart. Methods 1. The study of nerve cells was performed on 8 adult frogs Rana temporaria of both sexes. The experiments were performed in accordance to LSMU guidelines for the use of experimental animals. 2. Examples of flattened hearts were prepared to dissect the atria including interatrial septum, venal sinus, anterior caval veins and atrioventricular region in single piece after the heart was chemically fixed. 3. Cardiac nerve structures were stained immunohistochemically for choline acetyltransferase (ChAT) and tyrosine hydroxylase (TH) in flattened frog heart preparations. ChAT and TH are used as markers of the cholinergic and adrenergic neurons, respecti.[...].
Type of document
type::text::conference output::conference proceedings::conference paper
Other Identifier(s)
(LSMU ALMA)990000875860107106
Coverage Spatial
Lietuva / Lithuania (LT)
Language
Anglų / English (en)
Bibliographic Details
4
Affiliation(s)