Lithuanian University of Health Sciences Research Management System (CRIS)
Use this url to cite dataset: https://hdl.handle.net/20.500.12512/240992
microRNAs are abundant and stable in Platelet-rich fibrin and other autologous blood products of canines [Dataset]
Media Type (COAR)
DatasetMeasurement and test data
Author(s)
CreatorAffiliation
Jasinevičiūtė, Indrė  
VF Anatomijos ir fiziologijos katedra (13.03)  
Nazmul, Hasan Md.
Kagoshima University
Grigas, Juozas  
VF Anatomijos ir fiziologijos katedra (13.03)  
Pautienius, Arnoldas  
VF Anatomijos ir fiziologijos katedra (13.03)  
Stankevičius, Arūnas  
VF Anatomijos ir fiziologijos katedra (13.03)  
Naoki, Miura
Kagoshima University
Žymantienė, Judita  
VF Anatomijos ir fiziologijos katedra (13.03)  
Title
microRNAs are abundant and stable in Platelet-rich fibrin and other autologous blood products of canines [Dataset]
Art Work Nature
v1
Date Issued
2022-11-07
Keywords (en)
Source Publication
DOI
Abstract (en)

Regenerative properties and clinical adaptability of platelet-rich fibrin have been discussed in previous human research, but information about its composition in animals is lacking. It has been reported that microRNAs play an essential role in wound healing and inflammatory process. In this study, we reported the presence of various microRNAs in the platelet-rich fibrin and other autologous blood products of canines, including confirmed stability properties. We believe this research might be a step forward in understanding the regenerative mechanisms and adapting new therapy methods that can be used in humans and animals based on One Health principles

Is Referenced by
OpenAIRE
Geographic Coverage
Blood samples (n=20) from dogs (n=5) receiving treatment at the Kagoshima University Veterinary Teaching Hospital
Language
Anglų / English (en)
URI
URITitle
https://doi.org/10.5281/zenodo.7299985
Zenodo
https://hdl.handle.net/20.500.12512/240992
Affiliation(s)
VF Anatomijos ir fiziologijos katedra (13.03)  
VETERINARIJOS FAKULTETAS (13)  
Veterinarijos Akademija (VA)  
Lietuvos sveikatos mokslų universitetas (302536989)  
Funding(s)
NameID
Japan Society for the Promotion of Science KAKENHI
21H02366
Japan Society for the Promotion of Science KAKENHI
20K21375
Creative Commons License
http://creativecommons.org/licenses/by/4.0
Terms of Accessibility
Atviroji prieiga / Open Access
File(s)

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  • journal article[2023][S1][A002][10]
    Jasinevičiūtė, Indrė  
    ;
    Hasan, Nazmul
    ;
    Grigas, Juozas  
    ;
    Pautienius, Arnoldas  
    ;
    Stankevičius, Arūnas  
    ;
    Žymantienė, Judita  
    ;
    Miura, Naoki
    International Journal of Molecular Sciences, 2023-01-01, vol. 24, no. 1, p. 1-10

    Various microRNAs (miRNAs) present in autologous blood products of canines have not been studied recently. We aimed to elucidate the existence of miRNAs in platelet-rich fibrin (PRF) and the stability of canine autologous blood products under various storage conditions. Total RNAs were isolated from PRF and other autologous blood products following newly adapted protocols used in commercial kits for plasma and tissue samples. Quantitative real-time polymerase chain reaction analysis (qPCR) was used to detect miRNAs in autologous blood products. The miR-16, miR-21, miR-155, and miR-146a were abundant in PRF and other autologous blood products of canines. Furthermore, we found they could maintain stability under protracted freezing temperatures of −30 °C for at least one month. Our findings revealed that PRF might be a stable resource for various canine miRNAs.

      28WOS© Citations 4