- Physical and chemical analytical tests
- Testing of biological samples
- Production and testing of pharmaceutical dosage forms
- In vitro studies in mammalian cell culture/ex vivo models
- In vivo studies
- Food/food product testing
- Consultation
Analysis of metals and metalloids and their impact biomarkers in biomedia
Assessment of metals and metalloids and their impact biomarkers in human and animals biomedia in occupational health, environmental health, public health and clinical settings.
Ultra-performance liquid chromatography with mass spectrometry detector study
Analyses with a liquid chromatograph and Xevo TQD mass spectrometer (Waters, USA). The mass spectrometer works with ESI and APCI ion sources in MSscan, SIM, MS/MS modes. Applications: identity and quantitative analysis of organic compounds, determination of purity of materials.
Development, validation and application of an efficient liquid chromatography technique for the phytochemical analysis of objects of plant origin
Efficient liquid chromatography techniques are used for the analysis of phenolic, triterpene, diterpene and other specific metabolites.
Studies on medicinal substances, their metabolites and impurities
Identification and detection of the medicinal substance or its metabolite in complex samples of pharmaceutical, biological or other origin.
Studies on phenolic acids and flavonoids in plant samples
Ultra-performance liquid chromatography with mass spectrometry detector is used for the identification and determination of phenolic phytochemicals in plant samples and various products.
Qualitative and quantitative analysis of triterpenic compounds
The performance of the liquid chromatography assay is applied to the determination of triterpene compounds (ursolic, oleanolic, betulinic acid, etc.) for the analysis of samples of apples and their products.
Oxidised lipid tests
Ultra performance liquid chromatography with mass spectrometry detector for the determination of oxidised forms of lipids in biological samples. The test may be used for the determination of oxidation products of arachidonic acid metabolites.
Peptide and amino acid research
Ultra-performance liquid chromatography with mass spectrometry detector for the identification and determination of peptides and amino acids in biological samples. The method can be used for metabolism studies.
Determination of rosmarinic acid
The efficient liquid chromatography technique (according to Eur. Ph. 01/2011:1447) shall be used for the analysis of the quantity and identity parameters of rosmarinic acid-containing extracts of medicinal herbal raw materials.
Determination of total hydroxyanthracene glycosides and anthraquinones
The efficient liquid chromatography technique (according to Eur. Ph. 04/2020:0206) shall be used for the analysis of the quantity and identity parameters of extracts of medicinal plant materials containing hydroxyanthracene glycosides and anthraquinones.
Determination of total phenolic compounds
Total phenolic compounds are determined using the Folin-Ciocalteu spectrophotometric method.
Determination of total flavonoid content
The determination of total flavonoids in the test samples is based on the formation of complex compounds with Al3+ ions.
Determination of total proanthocyanidins
The total proanthocyanidins in the samples analysed are determined using the DMCA spectrophotometric technique.
Determination of total hydroxycinnamic acid derivatives
Determination of the total amount of hydroxycinnamic acid derivatives is based on reaction with Arnow’s reagent.
Determination of in vitro anti-radical activity
The in vitro anti-radical activity of the test samples is assessed by radical-binding spectrophotometric ABTS and DPPH methodologies and is expressed as a trolox equivalent per unit mass of sample.
Determination of in vitro reductive activity
The in vitro reductive activity of the test samples shall be assessed by spectrophotometric spectrophotometric determination of the reductive activity using the CUPRAC and FRAP methodologies and shall be expressed as trolox equivalent per unit mass of sample.
Assessment of the degree of oxidation in biological samples, antioxidant efficiency tests
Determination of the degree of oxidation of proteins, lipids and nucleic acids in biological samples.
Investigating the feasibility of optical detection of circulating metabolites in blood
Preparation of metabolite solutions, determination of concentrations in solutions and optimisation of measurement conditions. Measurement of metabolite concentrations in animal bloodstream by varying metabolite concentrations. Measurement of concentrations of multiple metabolites under conditions of artificial sepsis, comparison of results with laboratory results.
Determination of rheological properties
Evaluation of the rheological properties of semi-solid preparations (pharmaceuticals, cosmetics, foodstuffs): flow curve, oscillation, temperature test.
Particle size determination
Particle size of microemulsions, nanostructured carriers is determined by ZetaSizer; particles of emulsions, suspensions are determined by MasterSizer.
Determination of the pH of the sample
Biochemical analysis of blood
A blood test using a biochemical analyser. The concentration of various biochemical analytes in plasma or serum is determined.
Platelet aggregation test
A blood plasma test using the turbidimetric Born method. The luminal permeability of platelet-rich plasma stimulated with an inducer (adenosine diphosphate, epinephrine or arachidonic acid) is measured. The test is used to assess treatment with antiplatelet agents (e.g. clopidogrel, ticagrelor, prasugrel) or to assess platelet resistance to aspirin.
DNA isolation and concentration determination
Isolation of genomic DNA from peripheral blood leucocytes, saliva using the salt method or silica gel columns. DNA concentration and purity are determined spectrophotometrically.
Real-time PCR analysis of gene variants
The amount of the specific product of the resulting gene variant (e.g. CYP2C9*2*3, VKORC1 G-1639A, CYP2C19*2,*3,*17, CYP4F2*3, FV:Leiden, PAI-1 4G/5G, FII G20210A) produced shall be captured and measured using fluorescent markers and monitored in real time by real-time PCR.
Preparing sequencing libraries
Bacterial 16S rRNA sequencing libraries (to amplify the V1-V2 16S rRNA gene region), exome, transcriptome sequencing libraries, etc.
Determination of protein concentration
Co-concentration of various proteins in plasma, serum and other samples is determined by enzyme-linked immunosorbent assay (ELISA).
SARS-CoV-2 whole genome sequencing
Whole genome sequencing using Oxford Nanopore technology. The test involves scanning the genome of SARS-CoV-2 virus. The viral lineage and mutations are identified and sequenced in FASTA format. The test material is RNA from a nasopharyngeal sample.
Cell sorting and testing
Preparation, testing and sorting of cell samples using a cytometer-cell sorter. Expression studies for various surface antigens and receptors, intracellular protein expression studies, cell identification and quantitative analysis.
Cell imaging with the Stedycon superresolution microscope
Preparation of cell samples by immunofluorescence, imaging of samples. Live cell imaging using a temperature-controlled incubator and CO2 environment.
Manufacture of intermediate and final pharmaceutical forms
Formulation selection, technological functionalisation and stability studies of granules, microcapsules, tablets, capsules, syrups, solutions, potions, drops, ointments, gels, suppositories, etc.
Manufacture of cosmetic products
Formulation selection, technology and stability studies for various cosmetic products (creams, serums, hair and face masks, shampoos, conditioners, washes, mouthwashes, toothpastes, lipsticks, etc.).
In vitro biopharmaceutical studies of pharmaceutical forms
The dissolution kinetics of solid dosage forms (tablets, capsules, granules, powders and suppositories) shall be evaluated according to Ph. Eur. 2.9.3 and 2.9.42 monographs and other studies.
Modification of cell cultures and functional studies
Cells are transfected with siRNAs or miRNAs of the customer’s choice, and functional assays are performed in cell cultures (viability assays, colony formation, migration assays, etc.).
In vitro cytotoxicity assessment
Toxicity assessment of chemicals, biologicals and their preparations in cell culture. Parameters to be determined include: cellular metabolic activity, viability, death pathway, activation of the inflammatory response, oxidative stress, mitochondrial and glycolytic energy activity.
In vitro evaluation of anti-inflammatory effects
Evaluate the effect (efficacy) of agents and medical devices on viral (including COVID-19) or bacterial inflammation in vitro in a selected tissue model. Determine the production and secretion of immune and/or other markers of immunometabolic activity of selected cells induced by viral or bacterial agents, such as production and secretion of pro-inflammatory cytokines and chemokines, production of metalloproteinases, production of reactive oxygen and nitrogen species, mitochondrial-glycolytic energy metabolism transition, etc. Possible models include the respiratory tract, blood vessel, myocardium, gastrointestinal tract, etc.
Efficacy studies in an in vitro model of myocardial ischemia
Determine whether the investigational agent protects cardiomyocytes from ischemic death. The study may be extended to assess the effects on different death pathways (apoptosis, necrosis, ferroptosis, etc.), functionality (contractility) and mitochondrial damage.
Efficacy studies in an in vitro model of cerebral ischemia
Determine whether the substance being tested protects neurons from ischemia-induced death. Optional assessment parameters include neuronal death rate, number of synapses, synaptic activity and signal quality, level of inflammatory activity of astrocytes and microglia, mitochondrial and glycolytic functionality, and level of inflammatory factors.
Drug efficacy studies in an in vitro model of neurodegeneration
It assesses how the substance protects neurons (cultured together with other brain cells such as microglia and astrocytes) from neurotoxicity. Optional assessment parameters include neuronal death rate, number of synapses, synapse activity and signal quality, level of inflammatory activity of astrocytes and microglia.
Studies on effects on mitochondrial function
The effect of the product on the efficiency of the mitochondrial respiratory chain and phosphorylation system, the integrity of the inner and outer membranes, and other mitochondrial health parameters of the selected tissue is assessed.
Studies on effects on glycolytic activity
The effect of the product on the glycolytic efficiency of the selected tissue and the capacity of the glycolytic system is assessed.
Investigating the elicitation of an inflammatory response in human immune cells
Assess whether the test substances induce inflammation in immune cells such as macrophages, microglia, fibroblasts, astrocytes etc. The level of inflammation shall be assessed by the production and secretion of cytokines and chemokines, the production of metalloproteinases, the production of active oxygen and nitrogen compounds, the mitochondrial-glycolytic energy metabolism transition, etc.
Studies on anticancer effects
The effects of chemicals, biologicals and drugs in cell monolayers and three-dimensional (3D) cultures are investigated. Effects on viability, proliferation, migration, oncogenic factor production intensity and other parameters are determined.
In vitro/ex vivo multi-barrier permeation studies
It assesses the permeation of substances across various barriers: blood-brain, intestinal, vascular endothelial, skin, respiratory epithelial and other barriers in vitro.
Regenerative impact assessment
The regenerative effect on the cells of the selected tissue is evaluated using methods such as wound healing, total metabolic activity, energy metabolism intensity and others. Available models include skin (epidermis, mesoderm, hypodermis, full thickness), respiratory tract, brain, intestine, myocardium, skeletal muscle, retinal neuroepithelium, stem cells, immune cells, etc.
Evaluation of the effects on skin maintenance and functionality in a 3D in vitro model
The effect of the test substance on skin structure (layer thickness and shape, formation of characteristic layers) and function (production of extracellular filler proteins) is investigated in a 3D model of skin cultured in the air-liquid phase boundary.
Assessment of the maintenance of brain functionality in 3D in vitro models replicating natural cerebellar structure and functionality
Investigating neuronal viability and functionality by replicating the cellular composition, structure and function of the natural brain in 3D in vitro models.
Assessment of effects on osteogenesis
Effects on osteoblast viability, proliferation and differentiation are assessed.
Evaluation of the effects of substances in an in vitro model of osteonecrosis
The effect of the test substances on the viability, proliferation and energy metabolism of osteoblasts inhibited by bisphosphonates.
In vitro studies of the effects of neurodegenerative preparations in a model of Alzheimer’s disease
The models can be sporadic (based on amyloidogenic peptides), familial (based on mutation) or mixed. Determine whether the investigational agent protects neuronal synapses, improves their function, reduces inflammation and improves energy metabolism.
Ex vivo evaluation of the effects of medicinal substances or devices in the vasculature
Determine how the chosen drug or device affects the blood vessels ex vivo.
Ex vivo effects of ex vivo drug treatment on blood vessels by ultrasound modulation
Determine how the chosen drug or device affects the blood vessels ex vivo.
Isolation of lipocytes from lipoaspirate and assessment of their viability and determination of hemoglobin in lipoaspirate
Lipocyte viability/metabolic activity is assessed by fluorescence microscopy, Alamar blue and other methods, and hemoglobin content in the lipoaspirate is determined.
In vitro model of normal intestinal tissue
A 2D/3D in vitro intestinal model formed from colonic stem cells for testing various active substances. It can assess morphological changes, proliferation, apoptosis markers, changes in gene and protein expression, epithelial barrier function.
Toxicity and efficacy studies in in vivo models
According to the customer’s needs, the material is prepared for the study. Toxicity, pharmacokinetics, efficacy, bioanalysis of the material and surgical profile experiments are assessed. Cancer, infections, digestive system and other models are possible.
Effects on blood vessels in in vivo models
Determine how a selected drug or medical device affects blood vessels and their function in animal models.
Selection of microorganisms for the production of yeast bakery products
Selection of microorganisms for the production of leavening agents, selection of the optimum amount of leavening agent according to the sensory characteristics, porosity and specific volume of bakery products.
Quality tests on bakery products: porosity, specific volume, intensity of ringing during storage, sensory characteristics
The bakery products are analysed for porosity, specific volume, intensity of ringing during storage and sensory characteristics.
Modelling and/or optimisation of recipes for bakery and confectionery products
Formulation, selection of the optimum quantity of new ingredients and (if necessary) technological functionalisation.
Modelling fermented beverage technologies
Selection of microorganisms for the production of fermented beverages, optimisation of production conditions.
Modelling of chewing gum technology
Formulation, selection of the optimum quantity of new ingredients and (if necessary) technological functionalisation.
Modelling technologies for bioconversion of food industry by-products into valuable raw materials
Development of bioconversion schemes into valuable components for the food industry, according to the specificity of the by- product.
Determination of contamination of feed materials and feedstuffs with microscopic fungi (mould)
Assessment of contamination of feed materials and feedstuffs with fungi (number, genus).
Determination of mycotoxin concentrations in cereals and feeding stuffs
Mycotoxins: aflatoxins (total), aflatoxin B1, ochratoxins (total), ochratoxin A, deoxynivalenol, deoxynivalenol, zearalenone, T-2 toxin, T-2/HT-2 toxin, fumonisins B1+B2.
Determination of dry matter
The dry matter content shall be determined by weight on samples of natural moisture.
Determination of crude protein, crude fat, crude ash, crude fibre, starch
The analysis is carried out using the NIR Foss InfraXact spectrometer and other methods.
Determination of nitrogen and protein content
The study is carried out using the Kjeldahl method.
Determination of the moisture content of cereals and cereal products
The sample is analysed with a Kern MLB 50-3Ns moisture meter.
Determination of energy value (calorific value)
The test is carried out using an IKA C-2000 calorimeter and other methods.
Determination of organic matter in soil
Soil samples shall be analysed by the burn method.
Sensory evaluation of feed
Evaluation of hay, haylage, grain, silage by sensory and other means.
Oversight and management of studies
Experts from the University provide advice on study design, project strategy development and study protocols.
Training services
Training of the customer to independently and qualitatively carry out microbiological analyses of food, feed or water in accordance with the requirements of the ISO standard chosen by the customer.
Consultations for dental clinics
Consultancy services for private dental clinics (e.g. on dynamic navigation dental implants).
Advice for food processing companies
Advice on the application of microbiological criteria, experimental design and interpretation of results.
Consultancy services for the prevention of mycotoxicosis Health assessment of the dairy herd
Assessing the health of the herd, making recommendations.
Investigation and evaluation of the potential of different preparations for the health and reproductive success of fresh cows and calves and the prevention and treatment of clinical and subclinical mastitis in cows
