- Laboratory of Molecular Cardiology
- Cell Culture Laboratory
- Preclinical Research Laboratory for Medicinal Products
- Cardiac Pathology Laboratory
- Platelet aggregation test using adenosine diphosphate, epinephrine, or arachidonic acid
Platelet aggregation is performed in plasma using the conventional turbidimetric Born method. The light permeability of platelet plasma stimulated with an inducer (adenosine diphosphate, epinephrine, or arachidonic acid) is measured and expressed as a percentage. The test is used in the evaluation of antiplatelet/antiaggregant therapy (e.g. clopidogrel, ticagrelor, prasugrel) or in the evaluation of platelet resistance to aspirin.
Contact person: Vilius Skipskis, phone +370 37 302889
Price: 15 EUR
- DNA isolation, concentration measurement
Isolation of genomic DNA from peripheral blood leucocytes, saliva using the salt method or silica columns. The concentration and purity of the DNA is determined by measuring the light absorbance using a spectrophotometer.
Contact person: Ona Rūta Šereivienė, phone +370 37 302889
Price: 15 EUR
- Real-time PCR analysis of gene variants (reagents to be provided by the customer)
The amount of the specific product of the resulting gene variant is captured and measured using fluorescent markers and monitored in real time by real time PCR.
Contact person: Ieva Čiapienė, phone +370 37 302889
Price: 5 EUR
- Real-time PCR analysis of gene variants (CYP2C9*2*3, VKORC1 G-1639A, CYP2C19*2,*3,*17, CYP4F2*3, FV:Leiden, PAI-1 4G/5G, FII G20210A)
The amount of the specific product of the resulting gene variant is registered and measured using fluorescent markers and monitored in real time by real-time PCR.
Contact person: Ieva Čiapienė, phone +370 37 302889
Price: 15 EUR
- Biochemical analyser testing
The plasma or serum concentrations of various biochemical analytes can be determined using a biochemical analyser.
Contact person: Ona Rūta Šereivienė, phone +370 37 302889
Price: 5 EUR
- ELISA assay (reagents to be provided by the customer).
Concentrations of various proteins can be determined by enzyme-linked immunosorbent assay (ELISA). The test can be performed using blood plasma, serum, or other specimens.
Contact person: Ona Rūta Šereivienė, phone +370 37 302889
Price: 40 EUR
- SARS-CoV-2 whole genome sequencing
Whole genome sequencing method using the Oxford Nanopore technology. SARS-CoV-2 virus genome is read during the test. Result: virus variant, mutations identified, sequence presented in FASTA format. Analyte: RNA in nasopharyngeal swab.
Contact person: Dovydas Gečys, phone +370 37 302889; Lukas Žemaitis, phone +370 37 302889
Price: 97 EUR
- Cell sorting and testing with the BD FACSMelody™ cytometer-cell sorter.
Preparation, testing and sorting of cell samples using a cytometer-cell sorter. Expression studies of various surface antigens and receptors, expression studies of intracellular proteins, cell identification, and quantitative analysis.
Contact person: Ieva Sarapinienė, phone +370 37 302880; Valeryia Mikalayeva, phone +370 37 302880
Price: the price depends on the customer’s needs and specification of the services requested.
- Cell imaging with a Stedycon super-resolution microscope.
Preparation of cell samples by immunofluorescence, imaging of samples. Live cell imaging using a temperature-controlled incubator and CO2 environment.
Contact person: Ieva Sarapinienė, phone +370 37 302880; Valeryia Mikalayeva, phone +370 37 302880.
Price: the price depends on the customer’s needs and specification of the services requested.
- Toxicity studies of drugs, dietary supplements, functional foods in selected in vitro models
Assessment of cellular tolerance to the tested product. Possible assessment parameters include overall cell metabolic activity, viability (determination of LK50, selection of recommended doses for in vivo studies), determination of cellular death pathway, activation of the inflammatory response, oxidative stress, mitochondrial and glycolytic energy activity studies. Models implemented: gastrointestinal tract, respiratory tract, skin, nervous system, mucous membranes, blood vessels, cardiac and skeletal muscle, stem cells, immune cells. It is also possible to implement a customised model.
Contact person: Aistė Jekabsone, mobile phone number +370 675 94455
Price: 2,000–50,000 EUR (depends on the scope and complexity of the requested service)
- In vitro efficacy studies of drugs and medical devices for viral (including COVID-19) or bacterial inflammation in a selected tissue model
Anti-inflammatory drug efficacy studies by identification of markers of immunometabolic activity of immune and/or other selected cells induced by viral or bacterial agents, such as production and secretion of inflammatory cytokines and chemokines, production of metalloproteinases, production of reactive oxygen and nitrogen species, mitochondrial-glycolytic energy metabolism, and others. Possible models include the respiratory tract, blood vessels, myocardium, gastrointestinal tract etc.
Contact person: Aistė Jekabsone, mobile phone number +370 675 94455
Price: 2,000–500,000 EUR (depends on the scope and complexity of the requested service)
- Drug efficacy studies in an in vitro model of myocardial ischaemia
The studies are intended to verify the protective action of the investigational agent in relation to cardiomyocytes from the cell death caused by ischaemia. The study may be extended to assess the effects on different death pathways (apoptosis, necrosis, ferroptosis, etc.), functionality (contractility) and mitochondrial damage.
Contact person: Aistė Jekabsone, mobile phone number +370 675 94455
Price: 2000–25,000 EUR (depends on the scope and complexity of the requested service)
- Drug efficacy studies in an in vitro model of cerebral ischemia
The studies are intended to verify the protective action of the investigational agent in relation to neurons from the cell death caused by ischaemia. Optional assessment parameters include neuronal death rate, number of synapses, synaptic activity and signal quality, level of inflammatory activity of astrocytes and microglia, mitochondrial and glycolytic functionality, and level of inflammatory factors.
Contact person: Aistė Jekabsone, mobile phone number +370 675 94455
Price: 2,000–50,000 EUR (depends on the scope and complexity of the requested service)
- Drug efficacy studies in an in vitro model of neurodegeneration
Assessing how the product protects neurons (cultured together with other brain cells such as microglia and astrocytes) from neurotoxicity. Optional evaluation parameters include neuronal death rate, number of synapses, synaptic activity and signal quality, level of inflammatory activity of astrocytes and microglia.
Contact person: Aistė Jekabsone, mobile phone +370 675 94455
Price: > 2,000 EUR (depends on the scope and complexity of the requested service)
- Investigation of the effects on mitochondrial function
The investigation involves the assessment of the effect of the investigated product on the efficiency of the mitochondrial respiratory chain and phosphorylation system, the integrity of the inner and outer membranes, and other mitochondrial health parameters of the selected tissue.
Contact person: Aistė Jekabsone, mobile phone number +370 675 94455
Price: 2,000–50,000 EUR (depends on the scope and complexity of the requested service)
- Investigation of the effects on glycolytic activity
The investigation involves the evaluation of the effect of the product on the glycolytic efficiency and glycolytic capacity of the selected tissue.
Contact person: Aistė Jekabsone, mobile phone number +370 675 94455
Price: 2,000–50,000 EUR (depends on the scope and complexity of the requested service)
- Investigation of the elicitation of an inflammatory response in human immune cells
The investigation involves the assessment of whether the tested substances induce inflammation in immune cells such as macrophages, microglia, fibroblasts, astrocytes, etc. The level of inflammation is assessed by the production and secretion of cytokines and chemokines, production of metalloproteinases, production of active oxygen and nitrogen species, mitochondrial-glycolytic energy metabolism transition, etc.
Contact person: Aistė Jekabsone, mobile phone number +370 675 94455
Price: 2,000–20,000 EUR (depends on the scope and complexity of the requested service)
- In vitro blood-brain barrier crossing studies
Evaluation of the efficiency of the passage of compounds through the blood-brain barrier (consisting of endothelial and astrocyte layers) formed in vitro on a tissue culture insert membrane.
Contact person: Aistė Jekabsone, mobile phone number +370 675 94455
Price: 5,000–20,000 EUR (depends on the scope and complexity of the requested service)
- In vitro intestinal barrier crossing studies
Evaluation of the efficiency of passage of compounds through an intestinal barrier formed in vitro on a tissue culture insert membrane.
Contact person: Aistė Jekabsone, mobile phone number +370 675 94455
Price: 3,000–15,000 EUR (depends on the scope and complexity of the requested service)
- In vitro vascular endothelial barrier crossing studies
Evaluation of the efficiency of passage of compounds through a vascular barrier formed in vitro on a tissue culture insert membrane.
Contact person: Aistė Jekabsone, mobile phone number +370 675 94455
Price: depends on the scope and complexity of the requested service
- In vitro skin barrier crossing studies
Evaluation of the efficiency of passage of compounds through a skin barrier formed in vitro on a tissue culture insert membrane.
Contact person: Aistė Jekabsone, mobile phone number +370 675 94455
Price: 2,000–50,000 EUR (depends on the scope and complexity of the requested service)
- In vitro airway epithelial barrier crossing studies
Evaluation of the efficiency of the passage of compounds through an airway epithelial barrier formed in vitro on a tissue culture insert membrane. A bilayer model to simulate the alveolus of the airway epithelium and vascular endothelium may be designed.
Contact person: Aistė Jekabsone, mobile phone number +370 675 94455
Price: 5,000–30,000 EUR (depends on the scope and complexity of the requested service).
- Regenerative impact assessment
Assessment of the regenerative effect on selected tissue cells by wound healing, overall metabolic activity, energy metabolism intensity, and other methods. Available models include skin (epidermis, mesoderm, hypodermis, full thickness), respiratory tract, brain, intestine, myocardium, skeletal muscle, retinal neuroepithelium, stem cells, immune cells, etc.
Contact person: Aistė Jekabsone, mobile phone number +370 675 94455
Price: 2,000–50,000 EUR (depends on the scope and complexity of the requested service)
- Evaluation of the effects on skin maintenance and functionality in a 3D in vitro model
Investigation of the effect of the tested product on skin structure (thickness and shape of layers, formation of characteristic layers) and function (production of extracellular filler proteins) in a 3D skin model cultured in air-liquid phases.
Contact person: Aistė Jekabsone, mobile phone number +370 675 94455
Price: 10,000–70,000 EUR (depends on the scope and complexity of the requested service)
- Evaluation of the anticancer efficacy in 3D tumour models on substrates simulating natural extracellular matrix
Assessment of viability, proliferation, migration and oncogenic factor production intensity of in vitro generated tumour mimicking cancer cell spheroids under near-natural conditions. Possible models for glioblastoma, melanoma, intestinal carcinoma and other tumours.
Contact person: Aistė Jekabsone, mobile phone number +370 675 94455
Price: 2,000–30,000 EUR (depends on the scope and complexity of the requested service)
- Assessment of the maintenance of brain functionality in 3D in vitro models replicating natural cerebellar structure and functionality.
Investigating neuronal viability and functionality in 3D in vitro models replicating natural brain cellular composition, structure, and function.
Contact person: Aistė Jekabsone, mobile phone number +370 675 94455
Price: 10,000–70,000 EUR (depends on the scope and complexity of the requested service)
- Assessment of effects on osteogenesis
Assessment of effects on osteoblast viability, proliferation, and differentiation.
Contact person: Aistė Jekabsone, mobile phone number +370 675 94455
- Evaluation of drug efficacy in an in vitro model of osteonecrosis
Involves determination of the effects of the investigated preparation on the viability, proliferation and energy metabolism of osteoblasts inhibited by bisphosphonates.
Contact person: Aistė Jekabsone, mobile phone number +370 675 94455
Price: 3,000–20,000 EUR (depends on the scope and complexity of the requested service)
- Efficacy studies of neuroregenerative preparations in an in vitro model of Alzheimer’s disease
The models can be sporadic (based on amyloidogenic peptides), familial (based on mutation) or mixed. The studies involve the determination of whether the investigated preparation protects neuronal synapses, improves neuronal function, reduces inflammation, improves energy metabolism.
Contact person: Aistė Jekabsone, mobile phone number +370 675 94455
Price: 3,000–50,000 EUR (depends on the scope and complexity of the requested service)
- Assessment of the degree of oxidation in biological samples, investigation of antioxidant efficacy
Determination of the degree of oxidation of proteins, lipids and nucleic acids in biological samples.
Contact person: Aistė Jekabsone, mobile phone number +370 675 94455
Price: 1,000–10,000 EUR (depends on the scope and complexity of the requested service)
- Detection and quantification of selected compounds by high-performance liquid chromatography
The study involves detection of the substance of interest in the sample and calculation of the quantity.
Contact person: Aistė Jekabsone, mobile phone number +370 675 94455
Price: 2,000–50,000 EUR (depends on the scope and complexity of the requested service)
- Ex vivo evaluation of the effects of medicinal substances or devices in blood vessels
Determination of the ex vivo vascular effects of the chosen drug or device.
Contact person: Edgaras Stankevičius, mobile phone number +370 687 48989
Price: > 1,000 EUR (depends on the scope and complexity of the requested service)
- Evaluation of the effects of medicinal substances or devices on blood vessels in in vivo animal models.
Determination of the effects of the selected drug or device on the blood vessels and their functions in in vivo animal models.
Contact person: Edgaras Stankevičius, mobile phone number +370 687 48989
Price: > 1,000 EUR (depends on the scope and complexity of the requested service)
- Effects of drugs on ex vivo blood vessels by ultrasound modulation
Determination of the effects of the chosen medicinal product or device on the ex vivo blood vessels.
Contact person: Edgaras Stankevičius, mobile phone number +370 687 48989
Price: > 1,000 EUR (depends on the scope and complexity of the requested service).
| Procedure | Unit of measure | Price, EUR (VAT excl.) | ||
| HISTOLOGICAL TECHNIQUES | ||||
| FROZEN SECTIONS CUT BY CRYOTOME | ||||
| Frozen sections cut by cryosectioning (the first glass slide) | glass slide | 13.00 | ||
| Additional frozen serial sections, unstained, of a single specimen, cut at the same time | glass slide | 11.00 | ||
| Hematoxylin eosin (HE) staining of frozen sections | glass slide | 3.00 | ||
| Histochemical staining of frozen sections | glass slide | 15–60 | ||
| PARAFFIN SECTIONS | ||||
| Paraffin block production | block | 5.00 | ||
| Unstained sections cut from a single paraffin block | glass slide | 4.00 | ||
| Unstained serial sections cut from a single paraffin block during a single cutting procedure | glass slide | 3.00 | ||
| Hematoxylin eosin (HE) staining | glass slide | 8.00 | ||
| Histochemical staining | glass slide | 10.00–15,00 | ||
| TISSUE MICROARRAYS (TMA) | ||||
| TMA design, preparatory works | experiment | 70.00 | ||
| TMA production from the available/submitted paraffin blocks | block | 150.00–300.00 | ||
| Unstained sections cut from a single TMA | glass slide | 12.00 | ||
| Hematoxylin eosin (HE) staining | glass slide | 10.00 | ||
| Histochemical staining | glass slide | 10.00–20.00 | ||
| IMMUNIHISTOCHEMICAL (IHC) PREPARATION PRODUCTION WITHOUT INTERPRETATION | ||||
| IHC reaction by standard laboratory-implemented method and antibody | 1 marker/1 glass slide | 23.00 | ||
| IHC reaction by standard laboratory-implemented method and customer’s antibody | 1 marker/1 glass slide | 18.00 | ||
| IHC reaction implementation works against Ag of other species (non-human) | 1 marker | 550.00 | ||
| IHC reaction implementation works against human Ag | 1 marker | 463.40 | ||
| TMA IHC reaction by standard laboratory-implemented method and customer’s antibody | 1 marker/1 glass slide | 54.00 | ||
| TMA IHC reaction by standard laboratory-implemented method and antibody | 1 marker/1 glass slide | 62.00 | ||
| MICROPREPARATION SCANNING, DIGITAL IMAGE ANALYSIS (LIGHT FIELD MICROSCOPY, LFM) | ||||
| Light field micro-image scanning | glass slide | 15.00 | ||
| Application of digital image analysis methods to specific investigational needs | specific task | 200.00 | ||
| Digital image analysis using the tissue analysis system (pilot analysis) | per field of view (image) | 10.00 | ||
| Image annotation, TMA design | hour | 40.00 | ||
| Analysis of the digital image of the project using the tissue analysis system | specific task (depends on the scope) | 1,000.00–5,000.00 | ||
| Saving of the scanned images in an external data storage medium (provided by the service provider) | 1 service/1 external data storage medium (<1TB) | 100.00 | ||
| OTHER SERVICES | ||||
| Consultation by a researcher-pathologist in the preparation of research projects, selection of research methodology | hour | 62.00 | ||
| Microscopic specimen review and paraffin block sampling for tissue microarrays and other tests | preparation/block | 6.00 | ||
| Preparation of unstained sections for molecular tests | 6.00 | |||
| Consultation including extensive analysis of clinical and pathological data | consultation by 1 specialist on 1 case | 36.00 | ||
| Statistical analysis and interpretation (by a highly experienced researcher) | hour | 100.00 | ||
| Performance and interpretation of a statistical analysis (by a highly experienced software engineer) | hour | 50.00 | ||